LOCUS       40924_5279       12186 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway vector pB4GWnR DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa 
            T, Mano S.
  TITLE     Gateway Vectors for Simultaneous Detection of Multiple 
            Protein-Protein Interactions in Plant Cells Using Bimolecular 
            Fluorescence Complementation
  JOURNAL   PLoS ONE 11 (8), E0160717 (2016)
  PUBMED    27490375
REFERENCE   2  (bases 1 to 12186)
  AUTHORS   Mano S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2013) Contact:Shoji Mano National Institute for 
            Basic Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 12186)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12186)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2013) Contact:Shoji Mano National Institute for Basic 
            Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji, 
            Okazaki, Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     These colnes were obtained at our laboratory.
FEATURES             Location/Qualifiers
     source          1..12186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     341..357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        872..1217
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1239..1363
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1400..1430
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1484..2140
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2485..2787
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2831..2955)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2966..3463
                     /codon_start=1
                     /product="amino-terminal fragment of monomeric red
                     fluorescent protein 1"
                     /label=amino-terminal fragment of monomeric red fluore
                     /note="nmrfp1"
                     /protein_id="BAV44525.1"
                     /translation="MEQKLISEEDLMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGE
                     GRPYEGTQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKW
                     ERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYP
                     ED"
     CDS             2969..2998
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3485..3737
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3771..3787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3795..3811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3819..3849)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3864..3885)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3919..4171)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4564..5352)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5376..5559)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5765..5789)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6317..7105
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7352..7940
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8126..8266)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8610..8804)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8873..9937)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10374..11000)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      241 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      301 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      361 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      421 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      481 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      541 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      601 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      661 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      721 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      781 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      841 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      901 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      961 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1021 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1081 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1141 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1201 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1261 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1321 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1381 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1441 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1501 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1561 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1621 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1681 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1741 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1801 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1861 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1921 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1981 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     2041 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2101 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2161 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2221 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     2281 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2341 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2401 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2461 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2521 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2581 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2641 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2701 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2761 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2821 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2881 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2941 cttgtacaaa gtggttgata acagcatgga acaaaagcta atctccgagg aagacttgat
     3001 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
     3061 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3121 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     3181 gtcccctcag ttcacgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3241 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3301 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3361 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3421 gggctgggag gcctccaccg agcggatgta ccccgaggac taagcttaga gctcgaattt
     3481 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     3541 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     3601 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     3661 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     3721 atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg
     3781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc
     3901 gcctatttct gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
     3961 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
     4021 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
     4081 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
     4141 agaaacttta ttgccaaatg tttgaacgat cggggatcat ccgggtctgt ggcgggaact
     4201 ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt
     4261 cgccgccgac gccgttgatg tggacgccgg gcccgatcat attgtcgctc aggatcgtgg
     4321 cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc accttcgacc gcctgttccg
     4381 cagagatccc gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     4441 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     4501 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     4561 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     4621 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     4681 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     4741 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     4801 gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     4861 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     4921 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     4981 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     5041 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     5101 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     5161 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     5221 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     5281 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     5341 atcttgttca atcatgcgaa acgatccaga tccggtgcag attatttgga ttgagagtga
     5401 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
     5461 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
     5521 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa
     5581 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
     5641 ataacgtgac tcccttaatt ctccgctcat gatgaattca gaaataaatt cagcctaatt
     5701 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     5761 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     5821 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     5881 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     5941 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     6001 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctcg
     6061 ttcggttcgt aagctgtaat gcaagtagcg taactgccgt cacgcaactg gtccagaacc
     6121 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttc ttgttatgac
     6181 atgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     6241 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     6301 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     6361 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     6421 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     6481 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     6541 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     6601 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     6661 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     6721 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     6781 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     6841 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     6901 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     6961 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     7021 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     7081 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     7141 gcttcgccgg cgttaactca agcgattaga tgcactaagc acataattgc tcacagccaa
     7201 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     7261 tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7321 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7381 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7441 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7501 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7561 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7621 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7681 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7741 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7801 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7861 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7921 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7981 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8041 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8101 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     8161 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8221 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     8281 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8341 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8401 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     8461 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     8521 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     8581 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     8641 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     8701 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     8761 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     8821 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     8881 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     8941 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     9001 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     9061 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     9121 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     9181 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     9241 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     9301 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     9361 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     9421 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     9481 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     9541 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     9601 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     9661 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     9721 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     9781 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     9841 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     9901 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg
     9961 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
    10021 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    10081 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    10141 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    10201 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    10261 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    10321 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    10381 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    10441 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    10501 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    10561 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    10621 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    10681 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    10741 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    10801 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    10861 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    10921 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    10981 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    11041 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    11101 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    11161 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    11221 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    11281 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    11341 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    11401 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    11461 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    11521 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    11581 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    11641 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    11701 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    11761 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    11821 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    11881 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    11941 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    12001 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    12061 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    12121 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    12181 agtact
//
